Pu•biome

Notes

An obligately anaerobic bacterial strain (CTTWT) belonging to the family <a class="namesforlife" title="Lachnospiraceae - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4118" target="xrefwindow" rel="namesforlife-name">Lachnospiraceae</a> within the class <a class="namesforlife" title="Clostridia - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.3875" target="xrefwindow" rel="namesforlife-name">Clostridia</a> was isolated from an anoxic soil sample subjected to biological or reductive soil disinfestation. Cells of the strain were Gram-stain-positive, short rods with peritrichous flagella. The strain was saccharolytic and decomposed polysaccharides, chitin, xylan and &beta;-1,3-glucan. Strain CTTWT decomposed cell biomass and cell-wall preparations of an ascomycete plant pathogen, Fusarium oxysporum f. sp. spinaciae. The strain produced acetate, ethanol, H2 and CO2 as fermentation products from the utilized substrates. The major cellular fatty acids of the strain were C16 : 1&thinsp;&omega;7c dimethylacetal (DMA), C16 : 0 DMA and C18 : 1&thinsp;&omega;7c DMA. The closely related species of strain CTTWT based on the 16S rRNA gene sequences were species in the genus <a class="namesforlife" title="Anaerocolumna - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.28833" target="xrefwindow" rel="namesforlife-name">Anaerocolumna</a> with sequence similarities of 95.2&ndash;97.6 %. Results of genome analyses of strain CTTWT indicated that the genome size of the strain was 5.62&thinsp;Mb and the genomic DNA G+C content was 38.3&thinsp;mol%. Six 16S rRNA genes with five different sequences from each other were found in the genome. Strain CTTWT had genes encoding chitinase, xylanase, cellulase, &beta;-glucosidase and nitrogenase as characteristic genes in the genome. Homologous genes encoding these proteins were found in the genomes of the related <a class="namesforlife" title="Anaerocolumna - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.28833" target="xrefwindow" rel="namesforlife-name">Anaerocolumna</a> species, but the genomic and phenotypic properties of strain CTTWT were distinct from them. Based on the phylogenetic, genomic and phenotypic analyses, the name Anaerocolumna chitinilytica sp. nov., in the family <a class="namesforlife" title="Lachnospiraceae - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4118" target="xrefwindow" rel="namesforlife-name">Lachnospiraceae</a> is proposed for strain CTTWT (=NBRC 112102T=DSM 110036T).

Update: Ueki2021