Pu•biome

Notes

A strictly anaerobic bacterial strain (27-44T) was isolated from a stool specimen from an autistic child collected in PR China. The strain was Gram-stain-positive, non-motile, non-pigmented, non-spore-forming, and cells were oval to rod-shaped. Strain 27-44T grew at 20&ndash;40&thinsp;&deg;C (optimal at 37&thinsp;&deg;C) and at pH 6.0&ndash;10 (optimal at 6.0&ndash;8.0). The major polar lipids were one phospholipid, two glycolipids, two aminophospholipids and one unidentified lipid. The major cellular fatty acids of strain 27-44T were C16 : 0 and C17 : 0 2-OH. The end product of glucose fermentation was mainly butyric acid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 27-44T was a member of the genus <a class="namesforlife" title="Blautia - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.13065" target="xrefwindow" rel="namesforlife-name">Blautia</a> and phylogenetically closely related to <a class="namesforlife" title="Blautia obeum - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.26399" target="xrefwindow" rel="namesforlife-name">Blautia obeum</a> ATCC 29174T (with 97.8 % seque nce similarity). The genome of strain 27-44T was 3.5 Mbp with a DNA G+C content of 42.36&thinsp;mol%. A total of 3436 genes were predicted and, of these, 3133 genes were annotated by KEGG. On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons, strain 27-44T represents a novel species within the genus <a class="namesforlife" title="Blautia - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.13065" target="xrefwindow" rel="namesforlife-name">Blautia</a> , for which the name Blautia intestinalis sp. nov. is proposed. The type strain is 27-44T= CGMCC 1.5285T=NBRC 113774T

Update: Wang2021