Pu•biome

Notes

A novel strictly anaerobic chemoorganotrophic bacterium, designated Mahy22T, was isolated from sulfidic bottom water of a shallow brackish meromictic lake in Japan. Cells of the strain were Gram-stain-negative, non-motile and coccoid in shape with diameters of about 600&ndash;800 nm. The temperature range for growth was 15&ndash;37 &deg;C, with optimum growth at 30&ndash;32 &deg;C. The pH range for growth was pH 6.2&ndash;8.9, with optimum growth at pH 7.2&ndash;7.4. The strain grew with NaCl concentrations of 5% or below (optimum, 2&ndash;3%). Growth of the strain was enhanced by the addition of thiosulfate. The major cellular fatty acids were C16:0 and anteiso-C15:0. Respiratory quinones were not detected. The complete genome sequence of strain Mahy22T possessed a 1 885 846 bp circular chromosome and a 12 782 bp circular genetic element. The G+C content of the genome sequence was 30.1 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belonged to the family <a class="namesforlife" title="Acholeplasmataceae - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4812" target="xrefwindow" rel="namesforlife-name">Acholeplasmataceae</a> , class <a class="namesforlife" title="Mollicutes - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4612" target="xrefwindow" rel="namesforlife-name">Mollicutes</a> . The closest relative of strain Mahy22T with a validly published name was <a class="namesforlife" title="Acholeplasma palmae - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4827" target="xrefwindow" rel="namesforlife-name">Acholeplasma palmae</a> J233T with a 16S rRNA gene sequence similarity of 90.5%. Based on the results of polyphasic analysis, the name Mariniplasma anaerobium gen. nov., sp. nov. is proposed to accommodate strain Mahy22T, along with reclassification of some <a class="namesforlife" title="Acholeplasma - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4813" target="xrefwindow" rel="namesforlife-name">Acholeplasma</a> species into Alteracholeplasma gen. nov., Haploplasma gen. nov. and Paracholeplasma gen. nov.

Update: Watanabe2021