Pu•biome

Notes

Six novel facultatively anaerobic, Gram-stain-positive, rod-shaped, non-haemolytic bacteria (zg-320T/zg-336, zg-917T/zg-910 and zg-913T/zg-915) isolated from animal tissues and human faeces were found to belong to the genus <a class="namesforlife" title="Corynebacterium - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6188" target="xrefwindow" rel="namesforlife-name">Corynebacterium</a> based on the phylogenetic analyses of 16S rRNA gene and 262 core genes set. Based on the greatest degree of 16S rRNA similarity, zg-320T/zg-336 had the highest 16S rRNA gene similarity to <a class="namesforlife" title="Corynebacterium falsenii - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6215" target="xrefwindow" rel="namesforlife-name">Corynebacterium falsenii</a> DSM 44353T (97.51 %), zg-917T/zg-910 to <a class="namesforlife" title="Corynebacterium coyleae - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6210" target="xrefwindow" rel="namesforlife-name">Corynebacterium coyleae</a> DSM 44184T (98.68 %), and zg-913T/zg-915 to <a class="namesforlife" title="Corynebacterium afermentans - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6192" target="xrefwindow" rel="namesforlife-name">Corynebacterium afermentans</a> subsp. <a class="namesforlife" title="lipophilum - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6192" target="xrefwindow" rel="namesforlife-name">lipophilum</a> CIP 103500T (98.79 %). The three novel type strains had a relatively high DNA G+C content (61.2&ndash;64.4 mol%), low DNA relatedness and ANI values with their respective neighbours: 23.5/72.7 %, 25.0/72.3%and 22.6/73.1 % (zg-320T vs. <a class="namesforlife" title="Corynebacterium auriscanis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6201" target="xrefwindow" rel="namesforlife-name">Corynebacterium auriscanis</a> CIP 106629T, <a class="namesforlife" title="Corynebacterium resistens - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.9673" target="xrefwindow" rel="namesforlife-name">Corynebacterium resistens</a> DSM 45100T and <a class="namesforlife" title="Corynebacterium suicordis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6271" target="xrefwindow" rel="namesforlife-name">Corynebacterium suicordis</a> DSM 45110T); 24.4/82.3% and 23.7/81.3 % (zg-917T vs. <a class="namesforlife" title="C. coyleae - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6210" target="xrefwindow" rel="namesforlife-name">C. coyleae</a> DSM 44184T and <a class="namesforlife" title="Corynebacterium jeddahense - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.25950" target="xrefwindow" rel="namesforlife-name">Corynebacterium jeddahense</a> JCBT); 26.8/83.7% and 27.7/84.4 % (zg-913T vs. <a class="namesforlife" title="Corynebacterium mucifaciens - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6247" target="xrefwindow" rel="namesforlife-name">Corynebacterium mucifaciens</a> ATCC 700355T and <a class="namesforlife" title="C. afermentans - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6192" target="xrefwindow" rel="namesforlife-name">C. afermentans</a> subsp. <a class="namesforlife" title="lipophilum - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6192" target="xrefwindow" rel="namesforlife-name">lipophilum</a> CCUG 32105T). The three novel species had C16 : 0, C18 : 0, C18 : 1 &thinsp;&omega;9c and C18 : 0 ante/C18 : 2 &thinsp;&omega;6,9c as the major cellular fatty acids; MK-8(H2) in strain zg-917T and MK-9(H2) in strains zg-320T and zg-913T were found to be the major respiratory quinones. For the three novel species, the detected major polar lipids included diphosphatidylglycerol, phosphatidyl inositol mannoside, phosphatidylglycerol and phosphatidylinositol, the cell-wall peptidoglycan was based on meso-DAP, and the whole-cell sugars mainly included ribose, arabinose and galactose. The three novel species grew optimally at 35&ndash;37&thinsp;&deg;C, 0.5 % (w/v) NaCl and pH 7.0&ndash;8.0; notably, they were tolerant of 10.5 % (w/v) NaCl. Based on the results of these comprehensive analyses, three novel species in the genus <a class="namesforlife" title="Corynebacterium - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.6188" target="xrefwindow" rel="namesforlife-name">Corynebacterium</a> are proposed, aptly named Corynebacterium zhongnanshanii sp. nov. (zg-320T = GDMCC 1.1719T = JCM 34106T), Corynebacterium lujinxingii sp. nov. (zg-917T = GDMCC 1.1707T = JCM 34094T) and Corynebacterium wankanglinii sp. nov. (zg-913T = GDMCC 1.1706T = JCM 34398T).

Update: Zhang2021